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Antibodies certain to the blocking peptide no longer bind into the epitope about the target protein. By evaluating the staining from your blocked antibody vs . the antibody by itself, you can see which staining is distinct.

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This guideline outlines further more achievable optimization of mobile and immunoassay parameters to make sure the absolute best results are...

This tutorial outlines further achievable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...

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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the images or one-way links will redirect you to a web site hosted by BenchSci that provides third-occasion scientific content material. Neither the written content nor the BenchSci technological know-how and processes for choice are actually evaluated by us; we're furnishing them as-is and without having guarantee of any type, including for use or software from the Thermo Fisher Scientific merchandise introduced.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...

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Blocking peptides are peptides that bind precisely to the concentrate on antibody and block antibody binding. These peptide ordinarily contains the epitope regarded via the antibody.

Concentrate on Information and facts Cyclin E belongs for the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and features as a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress as a result of S period.

Overexpression of this gene has long been noticed in lots of tumors, which leads to chromosome instability, and therefore may add to tumorigenesis. This protein was discovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial job in marketing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two added splice variants were being documented but in-depth nucleotide sequence details is not really however readily available.

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